Mix-n-Stain Biotin Antibody Labeling Kit (20-50mug),

Application

The Mix-n-Stain biotin antibody labeling kit can be used to attach this important hapten to biomolecules of interest for subsequent signal amplification detection with enzyme or fluorescent labeled streptavidin, avidin or neutravidin. The kit is suitable for labeling commercially available primary antibodies, either directly or after a simple antibody clean-up step. After labeling, the biotin is covalently linked to the antibody.

Components

The kit consists of 1 vial each of Mix-n-Stain Dye, Mix-n-Stain Reaction Buffer (10x), Mix-n-Stain Storage Buffer, and an ultrafiltration vial.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Mix-n-Stain Biotin dye antibody labeling kits dramatically simplify the process of preparing biotin labeled antibodies, particularly primary antibodies. Simply mix your antibody with biotin reagent in the buffer provided, a step that takes less than 30 seconds of hands-on time. After waiting for another 30 minutes, without a separation step, you will have a biotin labeled antibody conjugate. There is no need to calculate how much biotin reagent you should use; just mix your antibody with the entire amount of biotin provided and you will always produce optimal labeling. Moreover, the labeling reaction can tolerate the presence of common stabilizers, such as sodium azide, Tris, glycerol and low level of BSA.

General description

This labeling kit is for labeling 20-50 µg antibody with our proprietary biotin reagent.Mix-n-Stain Biotin Antibody Labeling Kit contains everything you need to rapidly label an antibody with biotin for secondary detection using an enzymatically or fluorescently labeled anti-biotin antibody or streptavidin.After labeling, biotin is covalently linked to the antibody with a degree of labeling of 4–6 biotin molecules per antibody molecule.Multiple antibodies labeled with biotin or CF^™ dyes using different Mix-n-Stain kits can be used for multicolor fluorescence staining without transfer of biotin or dyes between antibodies.

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer’;s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: [email protected].

Mix-n-Stain is a trademark of Biotium, Inc.

CF is a trademark of Biotium, Inc.